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Peigen Huang
Assistant Professor, Radiation Oncology

Huang Lab Research

Spontaneous Tumors and their Isograft Models: Histopathology & Pathophysiology Studies

Our research focuses on the development of spontaneous tumors in mice and on how the age, genetic background, and immune status of the mice-as well as the presence or absence of specific genes-affect the histopathological characteristics of the tumors.
We study the incidence of spontaneous tumors and their pathological pattern in the natural setting of aged mice, which are kept alive for nearly their full normal life span. The animals are raised within a gnotobiotic colony that is free of life shortening intercurrent infectious diseases. We have found a high incidence of subcutaneous sarcoma in our aging C3Hf/Sed female mice. In our aging, retired FVB/N breeder mice, tumors are most commonly found in the lungs. The incidence of spontaneous T-cell thymice lymphomas in severe combined immunodeficient (SCID) mice is strikingly high. We have also published the first comprehensive report of spontaneous nonthymice tumors, including 8 myoepitheliomas and 3 rhabdmoyosarcomas, from our SCID retired breeders recently. Our results show that the incidence of spontaneous tumors and their morphological changes are markedly strain dependent, and are immune status, as well as age-associated.
We are documenting the development, growth, and histopathological characteristics of spontaneous tumors in the GFP transgenic mice with FVB background (such as VEGF-GFP/FVB, and Tie2-GFP/FVB mice). Our goal is to test the hypotheses that (a) the insertion of GFP reporter genes affects the incidence of spontaneous tumors in aging FVB genotobiotic mice, as well as changes their pathological patterns, and (b) spontaneous tumors developed in FVB-GFP transgenic mice exhibit different biological and molecular biological characteristics, such as different growth and metastatic potential, and different GFP expression in tumors as compared to the tumors in wild-type FVB mice.
We are also interested in developing novel tumor lines that are derived from the spontaneous tumors found in our laboratory. These tumor lines are used to study tumor pathophysiology in specific strains of transgenic mice derived from the same genetic background as the spontaneous tumors. One of our tumor lines, Os-P0107, is derived from a spontaneous osteosarcoma in a VEGF-GFP transgenic mouse; each of the cells in an Os-P0107 tumor expresses green fluorescent protein (GFP), which makes them easy to locate and track with intravital microscopy.
Another tumor line, LAP0297, is a lung adenocarcinoma with a high incidence of distant lung metastases. This line, which is ideal for the study of metastasis, is derived from a spontaneous lung tumor in a FVB/N mouse. For pre-clinical studies of antiangiogenesis therapy, we have used spontaneous autochthonous tumors and their isografts, implanted in aged C3Hf/Sed mice, to more accurately simulate the clinical conditions that affect many human cancer patients.

Huang Lab Careers

Gnotobiotic Technologist

Investigator: Huang, Peigen
Date Posted: 2013-03-05
Description
We are looking for a Gnotobiotic Technologist to join our Surgical & Animal Core at Cox-7 Animal Facility, the Edwin L. Steele Laboratory. Cox-7 Animal Facility is a gnotobiotic animal colony operated by the Department of Radiation Oncology at the Massachusetts General Hospital. This unique animal facility allows us to carry out longitudinal tumor physiological studies in immunodeficient and genetically engineered mice. All animals are maintained in defined flora condition with a state of the art gnotobiotic animal handling procedures.
Responsibilities
The Gnotobiotic Technologist has the primary responsibility for the isolator breeding program and is also expected to assist maintenance of gnotobiotic mice and genetically engineered mice breeding colony.
Requirements
Bachelor degree in biology, microbiology, laboratory animal science or related field is required. Knowledge in Bacterology, Virology, and Animal Science are also required.
Individuals should have minimum of 2 years of laboratory experience.
Interested candidates should send a cover latter and resume to:
Peigen Huang, M.D., M.Sc.
Director, Cox-7 Animal Facility
Edwin L. Steele Laboratory
Massachusetts General Hospital
100 Blossom Street , Cox-7
Boston , MA 02114
E-mail: peigen@steele.mgh.harvard.edu

Animal Care Assistant

Investigator: Huang, Peigen
Date Posted: 2013-03-05
Description
We are looking for an Animal Care Assistant to join our Surgical & Animal Core at Cox-7 Animal Facility, the Edwin L. Steele Laboratory. Cox-7 Animal Facility is a gnotobiotic animal colony operated by the Department of Radiation Oncology at the MGH. This unique animal facility allows us to carry out longitudinal tumor physiological studies in immunodeficient and genetically engineered mice. All animals are maintained in defined flora condition with a state of the art gnotobiotic animal handling procedures.
Responsibilities
The Animal Care Assistant has the primary responsibility for take good care of the animals, daily maintenance of gnotobiotic mice in the breeding, stock or experimental rooms. The individual is also expected to assist in the preparation of water bottles, cages with bedding, and in washing room; also to help with the general housekeeping sanitation of the facility each day.
Requirements
High school diploma is required. Knowledge in biology and Animal care, with good communication skill are also required.
Individuals should have minimum of 1 year of working experience.
Interested candidates should send a cover latter and resume to:
Peigen Huang, M.D., M.Sc.
Director, Cox-7 Animal Facility
Edwin L. Steele Laboratory
Massachusetts General Hospital
100 Blossom Street , Cox-7
Boston , MA 02114
E-mail: peigen@steele.mgh.harvard.edu

Selected Publications (from total of 41)

Huang P, Westmoreland SV, Jain RK, Fukumura D
Spontaneous nonthymic tumors in SCID mice.
Comp Med. 2011;61(3):227-34 - PMID: 21819692 - PMCID: PMC3123755
Liu J, Liao S, Huang Y, Samuel R, Shi T, Naxerova K, Huang P, Kamoun W, Jain RK, Fukumura D, Xu L
PDGF-D improves drug delivery and efficacy via vascular normalization, but promotes lymphatic metastasis by activating CXCR4 in breast cancer.
Clin Cancer Res. 2011;17(11):3638-48 - PMID: 21459800 - PMCID: PMC3107920 - DOI: 10.1158/1078-0432.CCR-10-2456
Wang E, Feng Y, Zhang M, Zou L, Li Y, Buys ES, Huang P, Brouckaert P, Chao W
Toll-like receptor 4 signaling confers cardiac protection against ischemic injury via inducible nitric oxide synthase- and soluble guanylate cyclase-dependent mechanisms.
Anesthesiology. 2011;114(3):603-13 - PMID: 21270629 - PMCID: PMC3044772 - DOI: 10.1097/ALN.0b013e31820a4d5b
Izumi Y, di Tomaso E, Hooper A, Huang P, Huber J, Hicklin DJ, Fukumura D, Jain RK, Suit HD
Responses to antiangiogenesis treatment of spontaneous autochthonous tumors and their isografts.
Cancer Res. 2003;63(4):747-51 - PMID: 12591719
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